Study: CBD Reduced Bladder Pain and Inflammation in Animal Model of Interstitial Cystitis

A study published in the World Journal of Men’s Health reports that cannabidiol (CBD) significantly reduced bladder inflammation and pain while improving bladder function in an animal model of interstitial cystitis/bladder pain syndrome (IC/BPS), a chronic condition that can be difficult to treat. The research was conducted by scientists from The Catholic University of Korea, The Affiliated Hospital of Qingdao University, Ewha Womans University, Colorado State University, Tianjin Institute of Urology, and Peking University First Hospital. The team examined how CBD acts within the bladder to reduce inflammation and relieve pain associated with IC/BPS.

Using female Sprague-Dawley rats, researchers induced IC/BPS through cyclophosphamide injections and then administered CBD directly into the bladder at two different doses. The animals treated with CBD showed marked improvements in bladder stability, longer intervals between bladder contractions, and stronger detrusor muscle contractions compared with untreated IC/BPS rats. Pain sensitivity testing found that the higher CBD dose produced the most pronounced reduction in pain responses.

Tissue analysis revealed that CBD-treated animals had significantly less bladder inflammation, reduced mast cell infiltration, and lower levels of fibrosis. At the molecular level, CBD reduced expression of several key inflammatory markers, including TNF-α, COX-2 and IL-6, while also lowering TRPV1 levels associated with pain signaling. The researchers identified suppression of the TLR4/MyD88/pNF-κB pathway as a central mechanism underlying CBD’s anti-inflammatory effects.

Additional cell-based experiments showed that CBD protected bladder epithelial cells from injury, reduced apoptosis-related signaling, and improved cell migration, further supporting its protective role in bladder tissue.

The findings suggest that intravesical CBD may offer a targeted approach for reducing inflammation and neuropathic pain associated with IC/BPS, highlighting its potential as a future therapeutic option for bladder pain disorders.